Characteristics of Recurrent Positive RT-PCR Findings of Recovered Patients From COVID-19 in Wuhan, China: A Retrospective study (preprint)

After two month of the outbreak of corona virus disease 2019 (COVID-19) in Wuhan China, tens of thousands hospitalized patients, being recovered, little was known about the follow-up of the recovered patients. 758 recovered patients, hospitalized in 17 hospitals previously and quarantined in 32 rehabilitation post stations in Wuhan, China, were retrospectively reviewed for clinical characters, reverse transcriptase- polymerase chain reaction (RT-PCR) from throat swab specimens and serological COVID-19 rapid diagnostic test (RDT) for SARS-Cov-2. Among them, 59 patients (7.78%) had recurrent positive findings for COVID-19 on RT-PCR from throat swabs. Among the 50 patients which was effective samples for antibody detection, 45/50 (90.00%) and 4/50 (8.00%) patients had positive of IgG or dual positive of IgG/IgM RDT for COVID-19, respectively. A certain number of quarantined patients with COVID-19 had recurrent RT-PCR results of SARS-Cov-2, the possibility of transmission in the patients needs further investigation.

Detectable serum SARS-CoV-2 viral load (RNAaemia) is closely associated with drastically elevated interleukin 6 (IL-6) level in critically ill COVID-19 patients (preprint)

Background: Although the SARS-CoV-2 viral load detection of respiratory specimen has been widely used for novel coronavirus disease (COVID-19) diagnosis, it is undeniable that serum SARS-CoV-2 nucleic acid (RNAaemia) could be detected in a fraction of the COVID-19 patients. However, it is not clear that if the incidence of RNAaemia could be correlated with the occurrence of cytokine storm or with the specific class of patients. Methods: This study enrolled 48 patients with COVID-19 admitted to the General Hospital of Central Theater Command, PLA, a designated hospital in Wuhan, China. The patients were divided into three groups according to the Diagnosis and Treatment of New Coronavirus Pneumonia (version 6) published by the National Health Commission of China. The clinical and laboratory data were collected. The serum viral load detection and serum IL-6 levels were determined. Except for routine statistical analysis, Generalized Linear Models (GLMs) analysis was used to establish a patient status prediction model based on real-time RT-PCR Ct value. Findings: The Result showed that cases with RNAaemia were exclusively confirmed in critically ill patients group and appeared to reflect the illness severity. Further more, the inflammatory cytokine IL-6 levels were significantly elevated in critically ill patients, which is almost 10-folds higher than those in other patients. More importantly, the extremely high IL-6 level was closely correlated with the incidence of RNAaemia (R=0.902) and the vital signs of COVID-19 patients (R= -0.682). Interpretation: Serum SARS-CoV-2 viral load (RNAaemia) is strongly associated with cytokine storm and can be used to predict the poor prognosis of COVID-19 patients. Moreover, our results strongly suggest that cytokine IL-6 should be considered as a therapeutic target in critically ill patients with excessive inflammatory response.